Gai-GTP活性抗体/鼠单抗
| 型号: | Gai | | 货号: | 26901 | | 品牌: | NewEast Biosciences | | 参考价格: | 4800 | | 包装: | 30微升 | | 交货期: | 现货 |
包装与价格： | 订货号 | 纯度规格 | 包装 | 价格(元) | | 26901 | | 30微升 | 4800 |
产品名称 Gai-GTP antibody
Anti Gai GTP Mouse Monoclonal Antibody
产品介绍
| 产品全名: Anti-Gαi-GTP Mouse Monoclonal Antibody | | 应用: IP, IHC, IF | | 种属反应性的选择: Human, Mouse and Rat | | 物种/宿主: Mouse |
| 背景介绍: Heterotrimeric G proteins are critical cellular signal transducers. Gαi represents one sub-family of G proteins that could mediate the inhibition of adenylyl cyclases. Other biochemical and physiological functions of Gα i proteins are being explored. | | 免疫原: Recombinant full length protein of active Gα i1 | | 经过测试的应用: IP, IHC, IF | 推荐稀释比:
IP: 1 μg for 1~2 mg total cellular proteins
IHC, IF: 1:50-1:250 | | 浓度: 1 mg/ml |
| 成分: PBS (without Mg 2+ and Ca 2+ ), pH 7.4, 150 mM NaCl, 50% glycerol | | 种属反应性: Anti-active Gα i antibody recognizes active Gα i1, Gα i2, and Gα i3 of vertebrates | | 储存条件: Store at -20°C. Avoid repeated freezing and thawing | 免疫沉淀/蛋白质印迹:
Gαi activation assay.
A. CHO cells were transfected with wild-type Gα i1 (lanes 1 and 2) or constitutively active Gα i1-Q204L (lane 3). Cell lysates were treated with GDP(lane 1) or GTPγS (lane 3). Lysates were then incubated with an anti-active Gα i monoclonal antibody (Cat. No.26901) (top panel). The precipitated active Gα i was immunoblotted with an anti-Gα i monoclonal antibody (Cat. No. 26003). The bottom panel shows the Western blot with anti-Gα i monoclonal antibody (Cat.No. 26003) of the cell lysates.
B. HEK293 cells stably expressing human m2 mAChR were treated with (lane 2) or without (lane 1)carbachol. Cell lysates were then incubated with an anti-active Gα i monoclonal antibody (Cat. No. 26901) (top panel).The precipitated active Gα i was immunoblotted with an anti-Gα i rabbit polyclonal antibody (Cat. No. 21006). The bottom panel shows the Western blot with anti-tubulin of the cell lysates. |
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