| 描述: Anti-Sar1-GTP Mouse Monoclonal Antibody |
| 背景介绍: Small GTPases are a super-family of cellular signaling regulators. Sar1 is a member of these small GTPases. Sar1 functions as a molecular switch to control protein–protein and protein–lipid interactions that direct vesicle budding from the ER. |
| 免疫原: Recombinant full length protein of active Sar1a. |
| 经过测试的应用: IP, IHC, IF |
推荐稀释比:
IP: 1 μg for 1~2 mg total cellular proteins
IHC, IF: 1:50-1:250 |
| 成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
| 种属反应性: Anti-active Sar1 antibody recognizes active Sar1 of vertebrates. |
| 储存条件: Store at -20°C. Avoid repeated freezing and thawing |
免疫沉淀/蛋白质印迹:
Sar1 Activation Assay. Purified Sar1 proteins were loaded with GDP (lanes 2 and 3) or GTPγS (lanes 4 and 5). Immunoprecipitation was done with the anti-active Sar1 monoclonal antibody (Cat. No. 26916). Immunoblot was with an anti-Sar1 rabbit polyclonal antibody (Cat. No. 21090). |
