半乳糖凝集素3单克隆抗体

商品属性

产品介绍
英文名称Galectin 3 Mouse mAb

中文名称半乳糖凝集素3单克隆抗体

别    名LEG3_HUMAN; Galectin-3; LGALS3; Gal-3; 35 kDa lectin; Carbohydrate-binding protein 35; CBP 35; CBP35; Galactose-specific lectin 3; Galactoside-binding protein ; GALBP; IgE-binding protein; L-31; Laminin-binding protein; Lectin L-29; Mac-2 antigen; MAC2; galectin 3; L31; GAL3; GALIG;  

研究领域肿瘤  免疫学  细胞凋亡  

抗体来源Mouse

克隆类型Monoclonal

克 隆 号7E4

交叉反应Human,Rat

产品应用WB=1:500-2000,IHC-P=1:200-1000,IHC-F=1:200-1000,IF=1:200-1000

not yet tested in other applications.

optimal dilutions/concentrations should be determined by the end user.

理论分子量29 kDa

检测分子量

细胞定位细胞核 细胞浆 分泌型蛋白

性    状Liquid

浓    度1mg/ml

免 疫 原human Galectin 3 full length protein

亚    型IgG

纯化方法affinity purified by Protein G

缓 冲 液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

保存条件Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

注意事项This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

PubMedPubMed

产品介绍This gene encodes a member of the galectin family of carbohydrate binding proteins. Members of this protein family have an affinity for beta-galactosides. The encoded protein is characterized by an N-terminal proline-rich tandem repeat domain and a single C-terminal carbohydrate recognition domain. This protein can self-associate through the N-terminal domain allowing it to bind to multivalent saccharide ligands. This protein localizes to the extracellular matrix, the cytoplasm and the nucleus. This protein plays a role in numerous cellular functions including apoptosis, innate immunity, cell adhesion and T-cell regulation. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Apr 2010].

高价值克隆与功能特性

MONOSAN B2C10（小鼠单抗）：

表位定位：识别Galectin-3的N端前45个氨基酸（干扰蛋白自缔合，抑制其血球凝集活性和中性粒细胞超氧化物生成）；

交叉反应：与人、小鼠Galectin-3反应，不识别Galectin-3C片段（适合区分全长与剪切体）；

功能实验：可用于中和实验（阻断Galectin-3介导的细胞粘附），推荐稀释比：WB 1:500-1000，IHC-P 1:100-200。

InVivoMAb M3/38（大鼠单抗）：

亚型与优势：大鼠IgG2a，可用于体内中和实验（如抑制肿瘤转移或纤维化模型）；

应用场景：流式细胞术（FC）检测巨噬细胞表面Galectin-3，推荐浓度2-5μg/mL。

实验应用扩展

实验类型 关键参数

流式细胞术（FC） 样本需用4%多聚甲醛固定（避免细胞膜破坏），抗体稀释至1μg/100μL，4℃避光孵育30分钟

功能阻断实验 预孵育抗体浓度10-20μg/mL（37℃，1小时），可抑制Galectin-3与整合素β1的结合

免疫沉淀（IP） 每500μg总蛋白添加2-5μg抗体，4℃旋转孵育4小时，推荐使用Protein G磁珠

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