3-磷酸甘油醛脱氢酶（内参）抗体

商品属性

产品介绍
英文名称GAPDH Rabbit pAb, Loading Control

中文名称3-磷酸甘油醛脱氢酶（内参）抗体

别    名38 kDa BFA-dependent ADP-ribosylation substrate; Aging-associated gene 9 protein; BARS-38; cb609; EC 1.2.1.12; G3PD; G3PDH; GAPD; Glyceraldehyde 3 phosphate dehydrogenase; Glyceraldehyde 3 phosphate dehydrogenase liver; Glyceraldehyde 3 phosphate dehydrogenase muscle; KNC-NDS6; MGC102544; MGC102546; MGC103190; MGC103191; MGC105239; MGC127711; MGC88685; OCAS, p38 component; OCT1 coactivator in S phase, 38-KD component; wu:fb33a10.

产品类型内参抗体

研究领域肿瘤  细胞生物  免疫学  信号转导  新陈代谢  

抗体来源Rabbit

克隆类型Polyclonal

克 隆 号

交叉反应Human,Mouse,Rat (predicted: Hamster)

产品应用WB=1:5000-50000,IHC-P=1:200-1000,IHC-F=1:200-1000,IF=1:200-1000,ELISA=1:5000-10000

not yet tested in other applications.

optimal dilutions/concentrations should be determined by the end user.

理论分子量38 kDa

检测分子量

细胞定位细胞核 细胞浆 细胞膜

性    状Liquid

浓    度1mg/ml

免 疫 原Recombinant human GAPDH full length protein

亚    型IgG

纯化方法affinity purified by Protein A

缓 冲 液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

保存条件Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

注意事项This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

PubMedPubMed

产品介绍Glyceraldehyde 3 phosphate dehydrogenase (GAPDH) is well known as one of the key enzymes involved in glycolysis. As well as functioning as a glycolytic enzyme in cytoplasm, recent evidence suggests that mammalian GAPDH is also involved in a great number of intracellular proceses such as membrane fusion, microtubule bundling, phosphotransferase activity, nuclear RNA export, DNA replication, and DNA repair. During the last decade a lot of data appeared concerning the role of GAPDH in different pathologies including prostate cancer progression, programmed neuronal cell death, age related neuronal diseases, such as Alzheimer's and Huntington's disease. GAPDH is expressed in all cells. It is constitutively expressed in almost all tissues at high levels. There are however some physiological factors such as hypoxia and diabetes that increase GAPDH expression in certain cell types. GAPDH molecule is composed of four 36kDa subunits.

靶点与功能

GAPDH是糖酵解途径的关键酶，由4个36kDa亚基组成同源四聚体，在几乎所有组织和细胞中组成型表达（除成熟红细胞外）。其核心优势：

稳定性：mRNA和蛋白水平在大多数生理/病理条件下变化较小（如缺氧、营养缺乏时仍较稳定）；

适用性：广泛用于WB、IHC、IF等实验的内参校正，尤其适合总蛋白 loading 控制；

分子量：36kDa，与多数目的蛋白分子量区分度高（避免条带重叠）。

实验操作关键要点

WB内参校正：

上样量：推荐20-30μg总蛋白，确保GAPDH条带灰度值在线性检测范围内（避免过饱和）；

抗体稀释：兔单抗建议1:5000稀释（5%脱脂奶粉+TBST），4℃孵育2小时（无需过夜）；

灰度分析：使用ImageJ软件，目的蛋白灰度值需除以GAPDH灰度值后进行组间比较。

IHC-P组织染色：

阳性定位：所有有核细胞胞质弥漫性阳性（心肌、肝细胞等强表达，神经细胞弱表达）；

对照设置：避免选择坏死组织（GAPDH降解）或红细胞丰富区域（无表达）。

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