质粒名称：CROPseq-Guide-Puro
质粒编号：86708
质粒备注：

Makes CRISPR gRNAs detectable by single-cell RNA-seq. The gRNA is expressed as part of the puromycin resistance mRNA transcribed by RNA Pol II and in its functional form from the hU6 promoter.

载体名称：lentiGuide-Puro
完整质粒大小：10214
载体类型：哺乳动物表达，慢病毒，CRISPR
筛选标记：嘌呤霉素

原核抗性：氨苄青霉素，100μg/mL
培养温度：30℃
克隆菌株：NEB Stable
高拷贝/低拷贝：高拷贝

插入基因：EF1a-Puro-WPRE-hU6-gRNA
物种来源：Synthetic
启动子：hU6

克隆方法：Gibson Cloning
5'测序引物：TTGGGCACTGACAATTCCGT

质粒图谱：