质粒名称：CTGF-bio-His
质粒编号：52028
质粒备注：

Expresses full-length Connective tissue growth factor precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

载体名称：pTT3
载体大小：6618
完整质粒大小：7685
载体类型：哺乳动物表达

原核抗性：氨苄青霉素，100μg/mL
培养温度：37℃
克隆菌株：Top10
高拷贝/低拷贝：高拷贝

插入基因：CTGF
基因别名：Connective tissue growth factor precursor
物种来源：H. sapiens (human)
插入大小：1067
基因名称：CCN2 (a.k.a. CTGF, HCS24, IBP-8, IGFBP8, NOV2)
启动子：CMV
标签/融合蛋白：· rat CD4 d3+4 (C terminal on backbone) · enzymatic biotinylation sequence (C terminal on backbone) · His tag (C terminal on backbone)

克隆方法：限制性内切酶
5'克隆位点：NotI (not destroyed)
3'克隆位点：AscI (not destroyed)
5'测序引物：CMV-fwd

质粒图谱：