产品说明
应用
- 从体外翻译系统中去除内源性RNA
- RNA测序实验
核酸酶S7已用于核糖体分析的核糖核酸酶选择过程研究。
生化/生理作用
Nuclease S7 is an endonuclease that mainly cleaves single-stranded substrates. It also cleaves double-stranded DNA or RNA. It cleaves the 5′-phosphodiester bonds of RNA and DNA to yield 3′-mononucleotides and oligonucleotides. Activity of the enzyme is dependent on Ca2+ and can be completely inactivated by the addition of EDTA. Nuclease S7 cleavage is highly specific to AT rich region than GC on the DNA.
单位定义
一单位酶释放的足够量的酸溶性寡核苷酸可使A260增加1.0。
储存及稳定性
未开封试剂储存于2-8 ℃;1 mg/mL的稀释液则储存于-15 to -25 ℃。
其他说明
仅用于生命科学研究。不可用于诊断。
产品性质
| 质量水平 | 100 |
| 生物来源 | Staphylococcus aureus |
| 形式 | lyophilized |
| specific activity | ~15,000 U/mg |
| 包装 | pkg of 15,000 U |
| manufacturer/tradename | Roche |
| 最佳pH | 7.5 |
| 运输 | wet ice |
安全信息
| 象形图 | ![]() ![]() |
| 警示用语: | Danger |
| 危险声明 | H315 - H317 - H319 - H334 - H335 |
| 预防措施声明 | P261 - P264 - P280 - P284 - P304 + P340 + P312 - P342 + P311 |
| 危险分类 | Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - Skin Sens. 1 - STOT SE 3 |
| 靶器官 | Respiratory system |
| 储存分类代码 | 11 - Combustible Solids |
| WGK | WGK 1 |
| 闪点(F) | does not flash |
| 闪点(C) | does not flash |





