产品说明
一般描述
Polynuclotide phosphorlyase in spinach chloroplasts acts as a exonuclease and a poly(A) polymerase.
应用
Polynucleotide phosphorylase has been used in a study to discover that a major function of PNPase is the synthesis of CDP. It has also been used in a study to investigate the enzyme responsible for RNA 3′-tail synthesis in S. coelicolor.
生化/生理作用
Polynucleotide phosphorylase localizes to the intermembrane space of mitochondria and has a critical function in regulating mitochondrial homeostasis in human cells.
Polynucleotide phosphorylase (PNPase) is a bifunctional enzyme with a phosphorolytic 3′ to 5′ exoribonuclease activity and a 3′-terminal oligonucleotide polymerase activity.
单位定义
Supplied as a solution in 20 mM Hepes buffer pH 7.9, 0.1 mM EDTA, 2 mM DTT, 12.5 mM MgCl2, 60 mM KCl, 20% (w/v) Glycerol
One unit will polymerize 1.0 μmole of ADP, releasing 1.0 μmole of inorganic phosphate in 15 minutes, at pH 9.1 at 37 ℃.
基本信息
| 酶学委员会(EC)编号 | 2.7.7.8 ( BRENDA | IUBMB ) |
| MDL编号 | MFCD00131992 |
| NACRES | NA.54 |
产品性质
| 质量水平 | 200 |
| 重组 | expressed in E. coli |
| 描述 | Histidine tagged |
| 形式 | solution |
| specific activity | ≥500 units/mg protein |
| 分子量 | 85 kDa |
| 运输 | dry ice |
| 储存温度 | −70℃ |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| WGK | WGK 1 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
