位置:首页 > 产品中心
LoVo人结直肠癌细胞(白人)
产品介绍
目录号
Serial
SCSP-514
标识符
Identifier
CSTR:19375.09.3101HUMSCSP514
描述
Description
人结直肠癌细胞LoVo构建于1971年,来源于一名56岁的白人男性结直肠癌患者转移到左锁骨位置的一个肿瘤结节。CSAp和结肠抗原3(colon antigen 3)表达阴性。肿瘤基因c-myc、K-ras、H-ras、 N-ras、Myb, sis和fos表达阳性,但未检测到 N-myc表达。该细胞表达肿瘤特定的核基质蛋白CC-3和CC-4。每个批次均通过本库支原体检测,结果阴性。经本库STR检测结果无误,STR结果如下:D5S818: 11,13;D13S317: 8,11;D7S820:9.3,10,11;D16S539: 9,12;vWA: 17,18;THO1: 9.3;Amelogenin: X,Y;TPOX: 8,9;CSF1PO: 10,11,13,14
动物种别
Organism
人;白人
性别
Gender
男性,56岁
组织来源
Tissue and Cell Type
结肠;分离自转移灶:左锁骨上区域
形态
Morphology
上皮样,贴壁生长
培养基和添加剂
Complete Growth Medium and Culture Conditions
完全培养液配方(100 ml)
Ham's F-12K (Kaighn's) Medium (Invitrogen, 21127-022)  90 ml
FBS (Gibco)  10 ml
参考传代周期:2-3天
参考传代比例:1:3-1:5
参考换液频率:每周2-3次
冻存液配方:完全培养液95%,DMSO 5%
气相:空气,95%;二氧化碳,5%。温度:37摄氏度。
供应限制
Permits and Restrictions
A。仅供研究之用。
REFERENCEDrewinko B, et al. Further biologic characteristics of a human carcinoembryonic antigen-producing colon carcinoma cell line. J. Natl. Cancer Inst. 61: 75-83, 1978. PubMed: 276641Drewinko B, Yand LY. Restriction of CEA synthesis to the stationary phase of growth of cultured human colon carcinoma cells. Exp. Cell Res. 101: 414-416, 1976. PubMed: 964319Drewinko B, et al. Establishment of a human carcinoembryonic antigen-producing colon adenocarcinoma cell line. Cancer Res. 36: 467-475, 1976. PubMed: 1260746Trainer DL, et al. Biological characterization and oncogene expression in human colorectal carcinoma cell lines. Int. J. Cancer 41: 287-296, 1988. PubMed: 3338874Keesee SK, et al. Nuclear matrix proteins in human colon cancer. Proc. Natl. Acad. Sci. USA 91: 1913-1916, 1994. PubMed: 8127905Drewinko B, et al. Response of exponentially growing, stationary-phase, and synchronized cultured human colon carcinoma cells to treatment with nitrosourea derivatives. Cancer Res. 39: 2630-2636, 1979. PubMed: 445465Miranda L, et al. Isolation of the human PC6 gene encoding the putative host protease for HIV-1 gp160 processing in CD4+ T lymphocytes. Proc. Natl. Acad. Sci. USA 93: 7695-7700, 1996. PubMed: 8755538
广州伟伯科技有限公司
手机: 13533759269
联系人: 林生
相关产品:
| 首页 | 联系我们 | 会员服务 | 广告服务 | 友情链接 |
版权所有 CopyRight © 2008-2024 粤ICP备08119708号