人k轻链重组兔单抗

商品属性

产品介绍
英文名称Human Ig Kappa light chain Recombinant Rabbit mAb

中文名称人k轻链重组兔单抗

别    名Kappa Light Chain; IGKC; HCAK 1; HCAK1; Ig kappa chain C region; IGKC; Immunoglobulin kappa constant; Immunoglobulin kappa constant region; Immunoglobulin kappa light chain; Kappa 1 immunoglobulin light chain; Km; MGC111575; MGC62011; MGC72072; MGC88770; MGC88771; MGC88809; Human Ig/L-Chain Kappa; IGKC_HUMAN.  

研究领域细胞生物  免疫学  

抗体来源Rabbit

克隆类型Recombinant

克 隆 号9B1

交叉反应Human

产品应用WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500

not yet tested in other applications.

optimal dilutions/concentrations should be determined by the end user.

理论分子量11 kDa

检测分子量

细胞定位细胞膜 分泌型蛋白

性    状Liquid

浓    度1mg/ml

免 疫 原A synthesized peptide derived from human Human Ig kappa light chain: IgA from human colostrum(Sigma#I1010)

亚    型IgG

纯化方法affinity purified by Protein A

缓 冲 液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

保存条件Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

注意事项This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

PubMedPubMed

产品介绍Immunoglobulins belong to a group of related glyco proteins which make up 20% of serum proteins. Antigens and immunoglobulins react to confer immunity to individuals. Immunoglobulins have similar structures of two identical heavy chains and two identical light chains. Both the heavy chains and the light chains are divided into constant and variable regions. The constant regions have the same amino acid sequences between all the immunoglobulin classes. The variable regions have approximately 110 amino acids with high sequence variability. The amino acid sequence of the heavy chain determines the class of an immunoglobulin. The five types of immunoglobulin heavy chains are known as: IgG, IgA, IgM, IgD, and IgE. IgG is divided into four subclasses, and IgA is divided into two subclasses. In serum IgA and IgG are monomers with a single 4 polypeptide unit; while, IgM is a pen tamer. IgA may also form polymers.

Kappa light chain antibody can be used for the identification of leukemias, plasmacytomas and certain non Hodgkin's lymphomas. Kappa light chain contains one immunoglobulin like domain. The EU sequence has the INV allotypic marker, Ala 45 and Val 83. The ROY sequence has the INV allotypic marker, Ala 45 and Leu 83.

靶点与功能

人k轻链（κ light chain）是免疫球蛋白（Ig）的组成部分，与重链（H链）通过二硫键连接形成抗体分子，核心研究方向：

抗体工程：用于重组抗体的轻链分型、表达量检测及纯化质控；

B细胞疾病：多发性骨髓瘤中κ轻链型单克隆蛋白的鉴定（κ/λ比值异常）；

自身免疫病：抗核抗体（ANA）检测中κ轻链阳性自身抗体的筛查。

实验操作关键要点

ELISA检测（单克隆抗体效价测定）：

包被条件：5μg/mL抗原（重组κ轻链蛋白）包被96孔板，4℃孵育过夜；

样本稀释：待检抗体用含1% BSA的PBS按1:1000-1:128000梯度稀释，37℃孵育1小时；

显色控制：TMB底物显色10分钟，2M H₂SO₄终止反应，450nm读取OD值（阳性判断阈值：OD450>0.2且S/N>2.1）。

WB检测（重组抗体表达）：

样本处理：细胞培养上清经Protein A/G亲和纯化后，12% SDS-PAGE电泳（κ轻链分子量约25kDa）；

抗体孵育：一抗1:1500稀释（5%脱脂奶粉+TBST），4℃孵育过夜，HRP二抗（山羊抗兔）1:5000室温孵育1小时；

条带验证：与重链（50kDa）条带对比，确认κ轻链表达完整性。

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