Endoglycosidases GKE-5010B-2

生物制药 HPLC 分析->N-糖样品前处理和分析->内切糖苷酶

AdvanceBio N-聚糖酶-plus (PNGase F)，≥ 10 U/mL. 酶（PNGase F 的重组形式）通过在最内侧 GlcNAc 和 Asn 之间裂解，释放完整 N-糖。以 5 瓶 GKE-5010B 提供（5 x 400 mU，≥ 10 U/mL，1 mmol/L EDTA）。包括 5 瓶 N-糖酶反应缓冲液：100 mmol/L 磷酸钠 pH 7.5，0.1% 叠氮化钠；变性溶液：2% SDS、1 mol/L 2 -巯基乙醇；去垢剂溶液：15% 去垢剂；5x N-糖酶 Tris 反应缓冲液：50 mmol/L Tris-HCl pH 8.0。

pH 范围	7.5-9.5
单位	2000 mU
浓度	10 U/mL
酶单位定义	One unit is defined as the amount of enzyme required to catalyze the release of N-linked oligosaccharides from 1 µmole of denatured ribonuclease B per minute at pH 7.5 and 37°C.
酶应用	To obtain efficient deglycosylation of glycoprotein substrates under nondenaturing conditions, it is necessary to use a higher starting concentration of enzyme. N-Glycanase-plus and ULTRA (EDTA-Free) are supplied at ≥ 10 U/ml, and are recommended for all applications requiring deglycosylation of glycoproteins in the absence of denaturants. The high activity also allows smaller reaction volumes and shorter reaction times to be explored.
酶来源	Recombinant gene from Elizabethkingia meningoseptica, expressed in E. coli. The source organism was previously known as Chryseobacterium [Flavobacterium] meningosepticum [1]. Enzyme also known as PNGase F, peptide-N-glycosidase F, peptide-N4-(N-acetyl-β-glucosaminyl)asparagine amidase.
酶比活性	≥10 units/mg
酶特异性	Cleaves all N-linked complex, hybrid or high mannose oligosaccharides, unless a(1-3) core fucosylated, as in plant glycans. Asparagine must be peptide bonded at both termini. Phosphate, sulfate and sialic acid groups attached to the oligosaccharide do not affect cleavage. Endo F free. Highly concentrated, particularly useful for deglycosylation under native conditions.
酶配方	20 mM Tris HCl pH 7.5, containing 1 mM EDTA and 50 mM NaCl