Endoglycosidases GKE-5020B-5

生物制药 HPLC 分析->N-糖样品前处理和分析->内切糖苷酶

AdvanceBio N-聚糖酶-ultra（PNGase F，不含 EDTA），≥ 10 U/mL. 重组形式的 PNGase F，通过在最内侧的 GlcNAc 和 Asn 之间裂解，释放完整 N-糖。与 GlykoPrep 的配方相同，≥ 10 U/mL，不含 EDTA。以 5 x 400 mU 的 GKE-5020B 样品瓶提供。包括 5x N-糖酶反应缓冲液、变性溶液、去垢剂溶液。

pH 范围	7.5-9.5
体积	400 µL
分子量	~35,000 Daltons
单位	2000 mU
** pH 值	8.6
浓度	10 U/mL
酶单位定义	One unit is defined as the amount of enzyme required to catalyze the release of N-linked oligosaccharides from 1 µmole of denatured ribonuclease B per minute at 37°C, pH 7.5.
酶应用	To obtain efficient deglycosylation of glycoprotein substrates under non-denaturing conditions, it is necessary to use a higher starting concentration of enzyme. N-Glycanase PLUS and ULTRA (EDTA-Free) are supplied at = 10 U/ml, and are recommended for all applications requiring deglycosylation of glycoproteins in the absence of denaturants. The high activity also allows smaller reaction volumes and shorter reaction times to be explored.
酶来源	Recombinant gene from Elizabethkingia meningoseptica, expressed in E. coli. The source organism was previously known as Chryseobacterium [Flavobacterium] meningosepticum. Enzyme is also known as Endoglycosidase F2, endo-beta-N-acetylglucosaminidase. Enzyme also known as PNGase F, peptide-N-glycosidase F, peptide-N4-(N-acetyl-β-glucosaminyl)asparagine amidase.
酶比活性	≥10 units/mg
酶特异性	Cleaves all N-linked complex, hybrid or high mannose oligosaccharides, unless a(1-3) core fucosylated, as in plant glycans. Asparagine must be peptide bonded at both termini. Phosphate, sulfate and sialic, acid groups attached to the oligosaccharide do not affect cleavage. Endo F free. Highly concentrated, particularly useful for deglycosylation under native conditions.
酶配方	20 mM Tris-HCl, 50 mM NaCl (pH 7.5, EDTA-free formulation).