质粒名称：pUC119-gRNA
质粒编号：52255
质粒备注：

Can use a PCR template to assemble new desired guide RNA. Contains an Arabidopsis U6 promoter to drive guide RNA (targeting AtPDS3 gene target site 1) expression with a TTTTTT as terminator.

载体名称：pUC119
载体大小：3162
载体类型：CRISPR ; Plant expression

原核抗性：氨苄青霉素，100μg/mL
培养温度：37℃
克隆菌株：DH5alpha
高拷贝/低拷贝：未知

插入基因：guide RNA targeting AtPDS3
基因别名：PDS3
基因别名：phytoene desaturase 3
物种来源：A. thaliana (mustard weed)
基因名称：PDS3 (a.k.a. AT4G14210, DL3145C, FCAALL.28, PDE226, PDS, PHYTOENE DESATURASE, PIGMENT DEFECTIVE 226, phytoene desaturase 3)
启动子：Arabidopsis U6 polymerase III promoter

克隆方法：限制性内切酶
5'克隆位点：SacI (not destroyed)
3'克隆位点：SmaI (destroyed during cloning)
5'测序引物：5’ TGGAATTGTGAGCGGATA 3’
3'测序引物：5’ ATTAAGTTGGGTAACGCC 3'

质粒图谱：