质粒名称：pGfa2-nLac
质粒编号：53126
质粒备注：

Expression of LacZ driven by the human Gfa2 promoter. LacZ can also be replaced with the gene of interest for targeting transgene expression to astrocytes in mice.

载体名称：pUC18
载体类型：Mammalian Expression, Mouse Targeting

原核抗性：氨苄青霉素，100μg/mL
培养温度：37℃
克隆菌株：DH5alpha
高拷贝/低拷贝：未知

插入基因：Gfa2
基因别名：glial fibrillary acidic protein
基因别名：GFAP
物种来源：H. sapiens (human)
基因突变：contains -2163 to +47 (the human gfa2 segment), with the initiating ATG mutated to TTG
基因名称：GFAP (a.k.a. ALXDRD)
启动子：human GFAP
标签/融合蛋白：· NLS (C terminal on insert) · LacZ (C terminal on insert) · mP1 (C terminal on insert)

克隆方法：限制性内切酶
5'克隆位点：BglII (not destroyed)
3'克隆位点：BglII (not destroyed)
5'测序引物：MB-351 CATCGCCAGTCTAGCCCACTCCT
3'测序引物：MB-352 GACGTTGTAAAACGACGGCCAGT

质粒图谱：