质粒名称：pFGC-pcoCas9
质粒编号：52256
质粒备注：

A binary plasmid containing 35SPPDK:pcoCas9:NOS cassette for plant expression of Cas9 and multiple cloning sites for insertion of guide RNA

载体名称：pFGC-RCS
载体类型：CRISPR ; Plant expression
筛选标记：Basta

原核抗性：卡那霉素，50μg/mL
培养温度：37℃
克隆菌株：DH5alpha
高拷贝/低拷贝：未知

插入基因：35SPPDK:pcoCas9:NOS cassette
基因别名：Cas9
基因别名：plant codon optimized Cas9
物种来源：Synthetic
启动子：constitutive 35SPPDK promoter

克隆方法：限制性内切酶
5'克隆位点：StuI (not destroyed)
3'克隆位点：StuI (not destroyed)
5'测序引物：Sequencing primer for multiple cloning sites (from somewhere close to the EcoRI site): 5’ AATAAAAACTGACTCGGA 3’
3'测序引物：Sequencing primer for 35SPPDK:pcoCas9:NOS (from somewhere close to the 35SPPDK promoter): 5’ AGTTGGGTAACGCCAGGG 3’

质粒图谱：