质粒名称：pC0055-CMV-dPspCas13b-GS-ADAR2DD(E488Q/T375G)-delta-984-1090
质粒编号：103871
质粒备注：

dPspCas13b-ADAR2DD(E488Q/T375G) fusion that can be used to selectively edit adenosine to inosine in RNA when used in conjuction with a guide RNA. Cas13b is truncated to allow for expression by AAV

载体名称：pcDNA3.1
载体大小：5428
完整质粒大小：9525
载体类型：哺乳动物表达，CRISPR

原核抗性：氨苄青霉素，100μg/mL
培养温度：37℃
克隆菌株：DH5alpha
高拷贝/低拷贝：高拷贝

插入基因：PspCas13b
物种来源：Prevotella sp. P5-125
基因突变：Changed histidne 133 to alanine and histidine 1058 to alanine to remove RNAse activity. Lacks residues 984-1090.
插入大小：2949


质粒图谱：