质粒名称：pBad-EBFP2
质粒编号：14891
质粒备注：

Bacterial expression of EBFP2 blue fluorescent protein

载体名称：pBad/His B
载体大小：4100
载体类型：原核表达

原核抗性：氨苄青霉素，100μg/mL
培养温度：37℃
克隆菌株：DH5alpha
高拷贝/低拷贝：低拷贝

插入基因：EBFP2
基因别名：EBFP2 blue fluorescent protein
物种来源：evolved fluorescent protein
基因突变：The fluorescent protein was inserted between XhoI and EcoR1 of pBad/His B. There is an extra 'C' after XhoI to avoid the frameshift.
插入大小：720

克隆方法：限制性内切酶
5'克隆位点：XhoI (not destroyed)
3'克隆位点：EcoRI (not destroyed)
5'测序引物：ATGCCATAGCATTTTTATCC
3'测序引物：GATTTAATCTGTATCAGG

质粒图谱：