质粒名称：pAC149-pCR8-dCas9VP160
质粒编号：48221
质粒备注：

dCas9VP160 on Gateway donor vector pCR8/GW/TOPO. Note: This is not for expression. It has to be transferred to a gateway destination vector for expression

载体名称：pCR8/GW/TOPO
载体大小：2799
完整质粒大小：7442
载体类型：CRISPR ; Gateway Donor

原核抗性：Spectinomycin, 50 μg/mL
培养温度：37℃
克隆菌株：DH5alpha
高拷贝/低拷贝：未知

插入基因：dCas9(D10A;H840A) fusion with VP160 activation domain
基因别名：dCas9VP160
物种来源：H. sapiens (human), Synthetic
基因突变：D10A;H840A nuclease-deficient
插入大小：4643
标签/融合蛋白：· HA Tag (N terminal on insert) · VP160 (C terminal on insert)

克隆方法：限制性内切酶
5'克隆位点：EcoRI (not destroyed)
3'克隆位点：EcoRI (not destroyed)
5'测序引物：M13F
3'测序引物：M13R

质粒图谱：