质粒名称：MSP1673
质粒编号：65769
质粒备注：

Bacterial expression plasmid for S. thermophilus1 Cas9 & sgRNA (need to clone in spacer into BspMI sites): T7-humanSt1Cas9-NLS-T7-BspMIcassette-St1-sgRNA

载体名称：pACYCDuet-1
载体类型：Bacterial Expression, CRISPR

原核抗性：氯霉素25μg/mL
培养温度：37℃
克隆菌株：XL1 Blue
高拷贝/低拷贝：低拷贝

插入基因：mammalian codon-optimized Streptococcus thermophilus1 Cas9-NLS, and St1Cas9 gRNA
启动子：T7 (x2)

克隆方法：Gibson Cloning
5'测序引物：ACYC-DuetUP1
3'测序引物：oBK390 (5'-GCAAATTCGACCCGGTCGTCG-3')

质粒图谱：