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Saos-2人骨肉瘤细胞(2019年新引进)
产品介绍
目录号
Serial
SCSP-5057
标识符
Identifier
CSTR:19375.09.3101HUMSCSP5057
描述
Description
该细胞系来自11岁白人女性的组织。该患者医疗使用过 RTG, methotrexate, adriamycin, vincristine, cytoxan, and aramycin-C。该细胞在免疫抑制小鼠中不致瘤,但在半固体培养基中形成集落。在我库通过支原体检测为阴性。冻存时为P35代。经本库STR检测无误。STR鉴定结果为:Amelogenin: X;CSF1PO: 10;D13S317: 12,13;D16S539: 12,13;D5S818: 12;D7S820: 8,10;THO1: 6,9;TPOX: 8;vWA: 18
动物种别
Organism
性别
Gender
女性,11岁
组织来源
Tissue and Cell Type
形态
Morphology
上皮样,贴壁生长
培养基和添加剂
Complete Growth Medium and Culture Conditions
人骨肉瘤细胞Saos-2完全培养液配方(100 ml):
McCoy's 5a Medium (Invitrogen, 12330-031)          85 ml
FBS (Gibco)             15 ml
备注:Saos-2细胞系通常没有很多碎片。但是,它始终都会有一些漂浮物。培养基中的小黑点可能是碎片,这可能因为没有达到***培养条件。我们建议仔细核对培养基配方和培养条件。请在细胞融合之前进行传代培养,并定期更换培养基。
供应限制
Permits and Restrictions
A。仅供研究之用。
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