Exoglycosidases GK80045

生物制药 HPLC 分析->N-糖样品前处理和分析->外切糖苷酶

AdvanceBio Sialidase A 酶，51 kDa. 低摩尔分子量版本的 GK80040 唾液酸酶 A，适用于具有挑战性的消化。该酶水解寡糖、糖蛋白释放 α(2-3)-、α(2-6)-、α(2-8)-和 α(2-9)- 连接的 N-乙酰神经氨酸。也可释放 N -羟乙酰神经氨酸。如果常规唾液酸酶存在空间位阻问题，较低分子量的唾液酸酶有助于多聚糖去唾液酸化。如果认为较低分子量的唾液酸酶有助于多聚糖去唾液酸化，您也可以试试 88 kDa、66 kDa 和 51 kDa 唾液酸酶 A 进样器试剂盒 (GK80043)。 
唾液酸酶也被称为神经氨酸酶。”

体积	200 µL
分子量	51 kDa
** pH 值	6
浓度	5 U/mL
酶单位定义	One unit is defined as the amount of enzyme required to catalyze the release of 1 µmole of p-nitrophenol from p-nitrophenyl-α-D-N-acetylneuraminic acid per minute at 37° C, pH 5.5.
酶来源	Recombinant gene from Arthrobacter ureafaciens, expressed in E. coli. Sialidases are also known as neuraminidases.
酶比活性	≥ 5 units/mg
酶特异性	The enzyme releases a(2,3)-, a(2,6)-, a(2,8)-, and a(2,9)-linked N-acetylneuraminic acid from complex carbohydrates. The initial rate of hydrolysis of a(2,6) linkages is reported to be approximately twice that of a(2,3)-linked sialic acid however, in practice, this kinetic selectivity is of little consequence during extended incubations. Effective digestion of glycolipid substrates is facilitated by addition of a detergent, such as sodium taurodeoxycholate to the incubation. Sialidase A is capable of releasing N-glycolylneuraminic acid (Neu5Gc, NGNA) in addition to N-acetylneuraminic acid (Neu5Ac, NANA) [1], although similarly to other sialidases [2] the activity is lower toward Neu5Gc than Neu5Ac. The enzyme can also be used to remove sialic acid from gangliosides [3], glycosphingolipids (ceramide and oligosaccharide) with sialic acid. May be used for exoglycosidase sequencing with Sialidase S (GK80021), which is specific for a(2,3)-linked sialic acids. Sialidases are also known as neuraminidase.