质粒名称：pRS300
质粒编号：22846
质粒备注：

Backbone for expressing plant artificial miRNAs

载体名称：pBluescriptSK+
载体大小：2964
载体类型：Plant Expression, RNAi ; Plant gene inactivation

原核抗性：氨苄青霉素，100μg/mL
培养温度：37℃
克隆菌株：DH5alpha
高拷贝/低拷贝：高拷贝

插入基因：MIR319a
物种来源：A. thaliana (mustard weed)
插入大小：404
基因名称：MIR319a (a.k.a. AT4G23713, JAGGED AND WAVY, JAW, MICRORNA 319, MIR319, MIR319A, microRNA319A, p_MI0000544)

克隆方法：限制性内切酶
5'克隆位点：Sma1 (destroyed during cloning)
3'克隆位点：Sma1 (destroyed during cloning)
5'测序引物：T7
3'测序引物：T3

质粒图谱：