质粒名称：pLCA.66/2272
质粒编号：22733

载体名称：pBluescript KS+
载体大小：2969
载体类型：Cre/Lox ; BAC recombineering
筛选标记：Neomycin (select with G418) ; ganciclovir

原核抗性：氨苄青霉素，100μg/mL
培养温度：37℃
克隆菌株：DH5alpha
高拷贝/低拷贝：高拷贝

插入基因：Lox66-pU(delta)TK-EM7Neo-Lox2272
基因突变：This vector was designed for assembling gene targeting constructs using the BAC recombineering approach. The vector contains a puromycin-(delta)thymidine kinase fusion gene driven by the mouse phosphoglycerol kinase promoter (pUDTK) and a neomycin resistant gene driven by the bacterial EM7 promoter (EM7neo). These selectable markers are flanked by lox66 and lox2272 sites (Cre-recombinase recognition sequences) in a pBluescript KS+ backbone with a modified multi-cloning region
插入大小：3697

克隆方法：限制性内切酶
5'克隆位点：Kpn1 (not destroyed)
3'克隆位点：Kpn1 (not destroyed)
5'测序引物：T3
3'测序引物：T7

质粒图谱：