质粒名称：pHEE401
质粒编号：71286
质粒备注：

Egg cell-specific promoter-controlled expression of 3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target sequence (U6-26 promoter), Hyg resistance

载体名称：pCambia
载体类型：Plant Expression, CRISPR ; Plant binary vector
筛选标记：潮霉素

原核抗性：卡那霉素，50μg/mL
培养温度：37℃
克隆菌株：DH5alpha
高拷贝/低拷贝：高拷贝

插入基因：sgRNA scaffold
物种来源：Synthetic
启动子：U6-26p Arabidopsis U6 gene promoter

克隆方法：限制性内切酶
5'克隆位点：BsaI (not destroyed)
3'克隆位点：BsaI (not destroyed)
5'测序引物：M13-fwd

质粒图谱：