质粒名称：pET28a/Cas9-Cys
质粒编号：53261
质粒备注：

Expresses N-terminal His tag fused human codon-optimized Cas9 nuclease having C-terminal Cysteine

载体名称：pET28a
载体大小：5369
完整质粒大小：9542
载体类型：原核表达

原核抗性：卡那霉素，50μg/mL
培养温度：37℃
克隆菌株：DH5alpha
高拷贝/低拷贝：未知

插入基因：Cas9-Cys
物种来源：S. pyogenes
基因突变：Additional Cysteine is added to the C-terminal region of Cas9
插入大小：4173
启动子：T7 Promoter
标签/融合蛋白：· 6X His tag (N terminal on backbone) · HA epitope (C terminal on insert) · NLS (C terminal on insert)

克隆方法：限制性内切酶
5'克隆位点：Not I (not destroyed)
3'克隆位点：Not I (not destroyed)
5'测序引物：TAA TAC GAC TCA CTA TAG GG
3'测序引物：GCT AGT TAT TGC TCA GCG

质粒图谱：